The protesters, who included multiple religious and political figures, called for the expulsion of the US ambassador from Pakistan as they marched through the city on Monday.
On Sunday, more than 5,000 people rallied in the southern port city of Karachi to condemn the insult to Islamic values.
Outrage has also grown globally over the US-made movie that insults Prophet Mohammad (PBUH) followed by sacrilegious cartoons published by French weekly Charlie Hebdo on September 19.
Muslims in Iran, Turkey, Sudan, Egypt, Yemen, Tunisia, Bangladesh, Indonesia, Malaysia, Kashmir, Pakistan, India, Iraq, Gaza, Morocco, Syria, Kuwait, Nigeria, Kenya, Australia, Britain, the United States, France, Belgium, and some other countries have held many demonstrations to condemn the blasphemous film.
Angry protesters across the globe demand an apology of the US government over the anti-Islam movie.
The movie is said to have been made with the help of the Zionist donations totaling USD 5 million.